This genomic fragment has already used in molecular phylogenetic analyses of longidorids and has successfully delimited several species belonging to cryptic complexes (Pedram et al., 2012 Gutiérrez-Gutiérrez et al., 2013 Pedram et al., 2017 Zhao et al., 2017 Fouladvand et al., 2019). In the inferred Bayesian phylogenetic tree, the newly generated sequence and the original sequence of the type population formed a basal clade to some other selected sequences of the genus (Fig. It had a 69.3 to 80.7% identity with sequences available in the GenBank database (154-265 different nucleotides). This variation could be explained by intraspecies variation in this genomic region and is recently observed for several other longidorids and trichodorids ( Pedram et al., 2017 Fouladvand et al., 2019). It had a 99.6% identity to the same sequence of the type population and three different nucleotides were detected between both populations. mindanaoensis (accession number: MN071244) from Vietnam was 805 bp long. The D2–D3 sequence of our population of L. (A) Esophageal region (B) Anterior region (C) Posterior region (E) Vulval region (F) Vulva region, ventral view. Figure 1:įigure 1: Longidorus mindanaoensis ( Coomans et al., 2012) from Vietnam. Note: All measurements are in μm (except for L in mm) and in the form: mean ± s.d. Longidorus mindanaoensis ( Coomans et al., 2012) Longidorus mindanaoensis (Vietnamese population) Measurements of Longidorus mindanaoensis ( Coomans et al., 2012) from Vietnam and the Philippines. could be highly variable ( Coomans et al., 2012 Archidona-Yuste et al., 2016). (2016) showing the body length of Longidorus spp. The present observation is in accordance with the results of Coomans et al. mindanaoensis from Vietnam are smaller than the type population (Table 1), and therefore, related indices, including a, b are also relatively smaller compared with the data given for the type population. However, new morphometric data ranges were recorded for the species as follows: the females and males of the presently studied population of L. Some unique morphological and morphometric characters like the shape of lip region and amphidial fovea, the characters of the esophageal bulb (its small size and shape as well as the arrangement of glands nuclei) and the position of vulva delimiting the species well corroborated the identity of the species. 1, Table 1) are in agreement with the original description of the species by Coomans et al. The morphological characters and measurements of the Vietnamese population of L. The morphological comparisons revealed the studied population belongs to Longidorus mindanaoensis ( Coomans et al., 2012) that was recovered from the same habitat in the Philippines by Coomans et al. MrBayes 3.2.6 ( Huelsenbeck and Ronquist, 2001) in Geneious R11 ( was used to infer the Bayesian phylogenetic tree with 10 6 generations of Markov chains (4 runs, 20% burn-in) ( Nguyen et al., 2019). Multiple alignments were made using MUSCLE and Modeltest was used to select the best fit model in MEGA 6 ( Tamura et al., 2013). The newly obtained sequence was compared with previously submitted sequences into the GenBank database ( Altschul et al., 1997) using BLAST search. For molecular phylogenetic analysis, the D2–D3 expansion segments of LSU rDNA were amplified using the primers D2A and D3B (5′-ACAAGTACCGTGGGGAAAGTTG-3′ and 5′-TCGGAAGGAACCAGCTACTA-3′) ( De Ley et al., 1999). The measurements and preparing the microphotographs were performed using a Carl Zeiss Axio Lab.A1 light microscope equipped with a ZEISS Axiocam ERc5s digital camera ( Nguyen et al., 2017). The specimens were killed, fixed in TAF, and transferred to glycerin according to Seinhorst (1959). Nematodes were extracted from soil samples using the tray method ( Whitehead and Hemming, 1965). Currently, only one valid species ( Longidorus elongatus (de Man, 1876) Micoletzky, 1927) has been reported in association with peanut ( Arachis hypogaea L.) from Vietnam.ĭuring this study, a population of the genus Longidorus was recovered from saline sea sediments in a mangrove forest in Vietnam (GPS coordinates N: 8☃8′09.902′ E: 104☄4′31.178′). Besides their direct damages by direct feeding from root cells, some species could also transmit plant pathogenic viruses ( Taylor and Brown, 1997). (needle nematodes) are migratory ectoparasitic nematodes.
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